cd4 t lymphocytes  (Bio-Rad)

Journal: Scientific Reports

Article Title: Exploring the effect of canine cancer-associated fibroblasts on T cell dynamics through the CXCL12/CXCR4 axis modulated by TGF-β1

doi: 10.1038/s41598-025-16312-x

Figure Lengend Snippet: Effect of TGF-β1 on CXCR4 expression on T cells. ( A ) CXCR4 expression on CD3⁺CD4 + and CD3⁺CD8 + T cells after being cultured with the medium containing TGF-β1 (5 ng/mL) with or without the TGF-βRI inhibitor SB431542 (5 µM) for 5 days. Mean ± SD ( n = 3). Plots are gated on lymphocyte > single cells > Live cells > CD3 + cells > CD4 + or CD8 + cells . ( B ) CXCR4 expression on CD3⁺CD4 + and CD3⁺CD8 + T cells after being cultured with NF-CM or CAF-CM for 5 days. Mean ± SD (Control, n = 3; NF-CM, n = 5; CAF-CM, n = 8). * P < 0.05, *** P < 0.001, **** P < 0.0001.

Article Snippet: In addition, cells were stained with the following directly fluorochrome-conjugated anti-canine antibodies: FITC-conjugated mouse anti-dog CD3 (clone CA17.2A12; Bio-Rad Laboratories), PE-conjugated anti-CD4 antibody (clone YKIX302.9, Bio-Rad Laboratories) and APC-conjugated rat anti-dog CD8 (clone YCATE 55.9; Thermo Fisher Scientific).

Techniques: Expressing, Cell Culture, Control

Journal: Scientific Reports

Article Title: Unraveling the role of shrimp hydrolysate as a food supplement in the immune function and fecal microbiota of beagle dogs

doi: 10.1038/s41598-025-09942-8

Figure Lengend Snippet: T lymphocyte proliferation evaluated by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells; ( B ) Percentage of CD3 + CD8 + cells, from dogs fed control diet and experimental diet, that proliferated at least once when non-stimulated (None) or in response to recombinant antigen from Leptospira interrogans (LipL32) and concanavalin A (ConA). Bars correspond to mean plus standard error of the mean. * P < 0.05.

Article Snippet: The PBMC were washed with PBS and stained with anti-dog CD3 FITC-conjugate (Bio-Rad), anti-dog CD4 PE-Cy7-conjugate (eBioscience), and anti-dog CD25 Super Bright 436-conjugate (clone P4A10, 62-0250-42, eBioscience), protected from light, for 25 min at 4 °C.

Techniques: Flow Cytometry, Control, Recombinant

Journal: Scientific Reports

Article Title: Unraveling the role of shrimp hydrolysate as a food supplement in the immune function and fecal microbiota of beagle dogs

doi: 10.1038/s41598-025-09942-8

Figure Lengend Snippet: Intracellular cytokine measurement by flow cytometry. ( A ) Percentage of CD3 + CD4 + cells expressing interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and both cytokines; ( B ) Percentage of CD3 + CD8 + cells expressing IFN-γ, TNF-α and both cytokines; ( C ) Ratio of CD3 + CD4 + and CD3 + CD8 + cells calculated from the percentage of CD4 + and CD8 + T cells; ( D ) Percentage of CD3 + CD4 + CD25 + cells expressing Foxp3 from dogs fed control diet and experimental diet. Bars correspond to mean plus standard error of the mean. ** P < 0.01.

Article Snippet: The PBMC were washed with PBS and stained with anti-dog CD3 FITC-conjugate (Bio-Rad), anti-dog CD4 PE-Cy7-conjugate (eBioscience), and anti-dog CD25 Super Bright 436-conjugate (clone P4A10, 62-0250-42, eBioscience), protected from light, for 25 min at 4 °C.

Techniques: Flow Cytometry, Expressing, Control